Optimization of culture conditions for the production of exopolysaccharides from marine isolate <i>Citrobacter</i> sp.
نویسندگان
چکیده
Exopolysaccharide (EPS) samples were collected from the Mandapam camp, India. Morphological, biochemical characterization and 16S rRNA gene sequencing done candidate bacterium was confirmed as <i>Citrobacter</i> sp. EPS production observed bacteria in Malt medium broth (0.88 ± 0.04mg/ml). The optimal cultural conditions for follows: after 72 hours of incubation at pH 8.0 (0.75 0.1 mg/ml), temperature 40⁰C 100 rpm (0.90 mg/ml). In experimentation, arabinose (carbon sources) (1. 0.120 mg/ml) ammonium nitrate (nitrogen) (1.30 showed better results yield EPS. highest recorded Triton X-100 (surfactants) supplemented medium. obtained 2.5% sodium chloride added Then inoculum concentration maximizing best noticed 96 time.
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ژورنال
عنوان ژورنال: Natural Resources for Human Health
سال: 2023
ISSN: ['2583-1194', '2583-1194']
DOI: https://doi.org/10.53365/nrfhh/159497